Bacterial and eukaryotic replication have many similarities. Which of the following is not a true comparison?
A. Both form loops with the lagging strand template to accommodate the antiparallel nature of DNA.
B. Both incorporate a sliding clamp mechanism in their replication polymerase.
C. Both the primase of bacteria and Pol of eukaryotes are responsible for primer synthesis and only incorporate ribonucleotides.
D. Eukaryotic replication rates are slower than bacterial rates.
E. The Okazaki fragments generated in eukaryotes are shorter than those in bacteria.
Which of the following is not true of the site-specific recombination reaction catalyzed by tyrosine-class recombinases?
A. One recombinase subunit becomes covalently linked to the DNA through a phosphotyrosine bond.
B. The overall reaction proceeds with a minimal free-energy change.
C. The overall reaction requires four unique cleavage events.
D. Isomerization of the Holliday intermediates includes branch migration through the core sequence.
E. All of the choices given are true.
Sulfanilamide is an antibacterial drug that inhibits dihydropteroate synthetase, an enzyme that converts para-aminobenzoic acid (PABA) to folic acid (which is required for the synthesis of bacterial nucleic acids).
Sulfanilamide is a structural analog to PABA so most likely is a(n)______________ inhibitor of dihydropteroate synthetase.
A. analogic inhibitor
B. uncompetitive inhibitor
C. mixed inhibitor
D. competitive inhibitor
E. allosteric inhibitor
Which of the following is an important tool in transcriptomics?
A. RNA-Seq
B. microarrays probed with antibodies
C. linkage analysis
D. two-dimensional gel electrophoresis
E. mass spectrophotometry
The site-specific recombinases are similar to topoisomerases in what way?
A. They introduce supercoils.
B. They require ATP hydrolysis for their activity.
C. They promote phosphoryl group transfers in which the net change in free energy is close to zero.
D. They also assist in compaction of the DNA.
E. They mediate strand invasion reactions.
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