× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
o
5
4
m
4
b
4
x
4
a
4
l
4
t
4
S
4
m
3
s
3
New Topic  
rjblayz rjblayz
wrote...
Posts: 48
Rep: 0 0
11 years ago
How are induced pluripotent stem cells produced?
Read 400 times
1 Reply

Related Topics

Replies
wrote...
11 years ago
"...iPS cells are typically derived by transfection of certain stem cell-associated genes into non-pluripotent cells, such as adult fibroblasts. Transfection is typically achieved through viral vectors, such as retroviruses. Transfected genes include the master transcriptional regulators Oct-3/4 (Pouf51) and Sox2, although it is suggested that other genes enhance the efficiency of induction. After 3?4 weeks, small numbers of transfected cells begin to become morphologically and biochemically similar to pluripotent stem cells, and are typically isolated through morphological selection, doubling time, or through a reporter gene and antibiotic selection..."
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  901 People Browsing
 114 Signed Up Today
Related Images
  
 959
  
 128
  
 1147
Your Opinion
Which industry do you think artificial intelligence (AI) will impact the most?
Votes: 308