Top Posters
Since Sunday
A
6
j
6
c
5
m
5
C
5
d
5
s
5
n
4
i
4
d
4
d
4
J
4
Home Q & A Board Laboratory Help Introductory Courses
New Topic  
toney32 toney32
wrote...
Go to Answer
Posts: 43
Rep: 1 0
11 years ago
I need to set up a serial dilution that is a serial 1:10 dilution of a stock reagent.  It's performed in a 250 microliter volume, and the dilution must go down past 1:100,000.  Help!
Read 504 times
2 Replies
Replies
wrote...
#1
11 years ago
if i take 1 mL of the reagent and add 9mL of water, it is diluted to 1/10th of the original.

if i take 1 mL of this new dilution and add another 9mL pure water, it is now 1/100th of that original concentration.

repeat this to get what you need (so repeat the 1/10th dilution a total of 5 times)except instead of 1 and 9, you use 25 and 225 microliters each time
Answer accepted by topic starter
rizzamellarizzamella
wrote...
#2
Posts: 73
Rep: 1 0
11 years ago
Sign in or Sign up in seconds to unlock everything for free
1

Related Topics
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  985 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 152
  
 316
  
 3
Your Opinion
Who will win the 2024 president election?
Votes: 8
Closes: November 4
Previous poll results: Which industry do you think artificial intelligence (AI) will impact the most?